NZVRSU

EUQG

How Agilent Seahorse Xf Analyzers Work

Di: Henry

Agilent Seahorse XF cell culture plates have been developed specifically for XF Analyzers to maximize the sensitivity of the sensor cartridge measurements, in order to provide valuable functional and file metabolic data from living cells, isolated mitochondria, islets, spheroids, and tissue models. Seahorse XF cell culture plates are tissue culture treated for optimal adherence and

Agilent Seahorse XF HS Mini Analyzer | Agilent

Seahorse XF HS Mini Agilent Seahorse XF HS Mini Analyzers measure mitochondrial respiration and glycolysis as well as ATP production rate of live cells in an eight-well miniplate format. The quick and easy setup makes the Seahorse XF HS Mini Analyzer ideal for performing routine energy metabolism measurements of ex-vivo and other quantity-limited samples. Seahorse XFp Cell Culture Miniplates. Compatible with the Seahorse XF HS Mini and XFp Analyzers. Includes 12 Seahorse XFp Cell Culture Miniplates per pack in individually sealed, gamma-irradiated tubs. Seahorse XF-24 Analyzers measure OCR and ECAR of live cells in a 24-well plate format, Seahorse-XFe96 – in 96-well format. OCR and ECAR rates are key indicators of mitochondrial respiration and glycolysis and these measurements provide a systems-level view of cellular metabolic function in cultured cells.

The XF T Cell Metabolic Profiling kit is also integrated with Agilent Wave Pro and Agilent Seahorse Analytics software to simplify data analysis, visualization, and interpretation. Each assay kit contains sufficient materials for six full-plate tests and is available in two formats. Agilent cell analysis solutions include the Seahorse XF Analyzers, the widely recognized standard in real-time cell metabolism analysis. xCELLigence RTCA Analyzers provide real-time new pharma oriented workflow solutions impedance-based analysis of cell function, eSight Analyzers enable real-time cell imaging, and NovoCyte flow cytometers offer high-performance, easy-to-use benchtop flow cytometry. Go beyond what How Agilent Seahorse XF Analyzers Work Seahorse XF Analyzers measure oxygen consumption rate (OCR) and extracellular acidification rate (ECAR) of live cells in a multi-well plate, interrogating key cellular functions such as mitochondrial respiration and glycolysis.

Seahorse XFp Analyzer Software

Agilent Seahorse XF Analyzers provide metabolic data on energy production in live cells in real time. Agilent provides a range of sensor cartridges and cell culture plates specific to your application and cell type needs. Agilent Seahorse XF assay kits, reagents, and media provide turnkey and tested workflows.

The Agilent Seahorse Glycolytic Rate Assay provides a precise measure of glycolysis in live cells, revealing transient responses and rapid metabolic switches not discernible in end-point lactate assays. Run on Agilent Seahorse XF Analyzers, this assay quantifies proton efflux specific to glycolysis and should be performed using XF pH-ready media. Agilent XF consumables, including Seahorse XF FluxPaks and Seahorse XF microplates, are developed specifically for use with the Seahorse XFe/XFp Analyzers to simplify your assays and ensure reliability and consistency of results. Specialized plates

The Agilent Seahorse XF Cell Mito Stress Test measures key parameters of mitochondrial function by directly measuring the oxygen consumption rate (OCR) of cells on the Seahorse XFe and XF Pro Extracellular Flux Analyzers. Agilent Seahorse Wave Desktop software transforms complex cellular metabolism foundational knowledge about data into publishable results. Wave desktop is the assay design, data analysis, and file management software for all Agilent Seahorse XF Analyzers. The latest release of Wave Desktop provides new analysis capabilities in a redesigned interface that will streamline your XF workflow, regardless

Agilent Seahorse XF Pro analyzer is an enhanced metabolic assay platform that measures real-time energy flux, mitochondrial function, glycolysis, and substrate utilization in living cells, isolated mitochondria, 3D cultures, and microbes. Agilent Seahorse XF analyzers simultaneously measure the two major energy pathways of the cell — mitochondrial respiration and glycolysis — in live cells, in real time. Seahorse XF analyzers work with all cell types, including cell culture primary cells, cell lines, suspension cells, as well as islets, C. elegans, yeast, and isolated mitochondria. The XF Glycolytic Rate Assay and XF Glycolysis Stress Test are available as kits for XFe96, XFe24, or XFp analyzers. Both kits provide pre-calibrated, pre-tested compounds and are coupled with powerful report generator software, making it easy to generate functional metabolic data. These kits work best when used with Seahorse XF Media.

  • Seahorse XF Cell Mito Stress Test Kit User Guide
  • The Chemistry behind the assay
  • Agilent Seahorse XF Flex Analyzer Brochure

Agilent Seahorse XFe24 분석기는 live cell의 에너지 대사를 실시간으로 측정합니다. 이 24-well 대사 분석기는 미토콘드리아 호흡, 해당작용 및 ATP 생성을 측정함으로써 세포 대사 기능을 시스템 차원에서 보여줍니다.

The Agilent Seahorse XF real-time metabolic analyzer revolutionized the measurement of bioenergetics in live cells. This has provided the foundational knowledge about cell energy metabolism and its role in disease progression and therapeutic development. Building on our previous success, Agilent has developed a versatile system that expands real-time metabolic Glycolysis is how Cells get Work Done Glycolysis is a critical metabolic pathway used in both normal and disease‐associated processes such as T cell activation and cancer cell proliferation. When glycolysis‐derived lactate is exported from the cell, protons are also exported, making extracellular acidification rate a convenient indicator of glycolysis. Agilent Seahorse XF

Agilent delivers complete scientific solutions, helping customers achieve superior outcomes in their labs, clinics, business and the world they seek to improve. Agilent offers Seahorse XF Sensor Cartridges and Extracellular Flux cell culture plates, designed for Seahorse XF analyzers to support a variety of cell-based assays. Seahorse 消耗品专用于 Seahorse XF 分析仪，能够实现灵敏的动力学测量，以同时分析线粒体呼吸和糖酵解。 Seahorse XF FluxPaks 包括探针板、组织培养板、校准液和用于校准的工具板。 探针板包含固态传感器探针，用于检测细胞培养基中的氧和质子浓度。

The extracellular flux analyzer, also known as the Seahorse Extracellular Flux (XF) analyzer, was first introduced in 2006 by Seahorse Sciences and is extensively utilized in cellular metabolism research.

What is Seahorse XF Analyzer? The XF Analyzer is the first and only commercially available instrument for scientific research that simultaneously measures in real-time and in live cells the two major energy pathways of the cell: mitochondrial respiration and glycolysis. How does Seahorse Analyser work? The Agilent Seahorse XF ATP Real-Time rate Agilent Seahorse XF Analyzers provide metabolic data on energy production in live cells in real time. Pro 세포 분석기는 96 well Agilent provides a range of sensor cartridges and cell culture plates specific to your application and cell type needs. Agilent Seahorse XF assay kits, reagents, and media provide turnkey and tested workflows. Agilent Seahorse Analytics is the newest data analysis application for Agilent Seahorse XF Analyzer products. Seahorse Analytics is built in the cloud, meaning there is no software for you to install, update, or manage. Learn how Seahorse

The Agilent Seahorse XF Pro analyzer measures the rate of change of dissolved oxygen and pH in the media immediately surrounding living cells cultured in a microplate. Are your cells built to meet energy challenges? Agilent Seahorse XF assay kits—designed to work with your Agilent Seahorse XF analyzer—help you to answer these questions and more. Follow the journey below to reveal the bioenergetic phenotype and the mechanisms that drive the behavior of your cell models.

The Agilent Seahorse XF analyzer revolutionized the measurement of bioenergetics in live cells. Now, we’ve built upon that success with the Seahorse XF Pro. It features new pharma-oriented workflow solutions with improved precision, along with advanced experimental design and Agilent Seahorse XF analyzers enables robust measurements of mitochondrial activity, glycolysis, and ATP production rates in a microplate format. Agilent Seahorse XF Pro 세포 분석기는 96-well 형식으로 라이브 셀의 산소 소모율 (OCR), 양성자 유출률 (PER), 세포 외 산성화율 (ECAR)과 ATP 생성률을 측정 및 보고합니다.

Agilent Seahorse XF Analyzer XFe96 and XFe24 User Guide provides detailed information on experiment design, instrument control, data acquisition, data analysis, and file management software for Agilent Seahorse XF, XFe, and XFp Analyzers.

The Agilent Seahorse XF analyzer can be used to measure the flux through mitochondrial respiration and glycolysis. This information allows you to develop a functional hypothesis on the experimental cell’s behavior and metabolic state.